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anti src py416  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti src py416
    Anti Src Py416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/src+py416/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pmc12686823-54-21-47
    Average 96 stars, based on 928 article reviews
    anti src py416 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Defective N-glycosylation of IL6 induces metastasis and tyrosine kinase inhibitor resistance in lung cancer.
    Article Snippet: .. The following Ab were used for WB: IL6 (Santa Cruz, sc-32296; RRID:AB_627804); STAT3 (BD, 610189; RRID:AB_397588); STAT3pY705 (CST, 9131;RRID:AB_331757); SRC (CST, 2108; RRID_331137); SRC-pY416 (CST, 2101; RRID:AB_331697); YAP (CST, 4912; RRID:AB_2218911); YAP-pY357 (Abcam, ab62751; RRID:AB_956486); β-ACTIN (Millipore, MAB1501; RRID:AB_2223041); SLUG (Abcam, ab-27568; RRID:AB_777968); CLAUDIN1 (Life Technology, 374900; RRID:AB_2533323); E-CADHERIN (BD, 610181; RRID:AB_397581); N-CADHERIN (BD, 610921; RRID:AB_398236); α-SMA (Abcam, ab-5694; RRID:AB_2223021); AKT (CST, 4691; RRID:AB_915783); AKT-pY308 (CST, 4056; RRID:AB_331163); AKT-pY473 (CST, 9271; RRID:AB_329825); SOX2 (Santa Cruz, sc-365823; RRID:AB_10842165); ERK1/2 (Santa Cruz, sc-514302; RRID:AB_2571739); pERK (Santa Cruz, sc-7383; RRID:AB_627545); LAMIN A/C (Merck Millipore, 05-714; RRID:AB_309928); NANOG (GeneTex, GTX-627421; RRID:AB_11169335); OCT3/4 (Santa Cruz, sc-5279; RRID:AB_628051); LATS1 (CST, 3477; RRID:AB_2133513); pLATS (CST, 8654; RRID:AB_10971635); TAZ (Abcam, ab224239; RRID:AB_2889852); pTAZ (CST, 59971; RRID:AB_2799578); MST1 (CST, 3682; RRID:AB_2144632). ..

    Article Title: Calmodulin Directly Interacts with the Cx43 Carboxyl-Terminus and Cytoplasmic Loop Containing Three ODDD-Linked Mutants (M147T, R148Q, and T154A) that Retain α-Helical Structure, but Exhibit Loss-of-Function and Cellular Trafficking Defects
    Article Snippet: After re-plating and once colonies were formed, Western blot and immunofluorescence were used to screen the clones (α-Cx43, Sigma #6219, 1:10,000, MilliporeSigma, St. Louis, MO, USA). .. Detection reagents, manufacturers, and concentration ranges for all primary and secondary antibodies utilized in Western blot imaging are as follow: Pyk2 (Cell Signaling #3480S, 1:1,000, Danvers, USA), Pyk2 pY402 (Abcam #4800, 1:1,000, Cambridge, UK), PKCα (Cell Signaling #2056S, 1:1,000), PKCα pT638 (Abcam #32502, 1:5,000), Src (Cell Signaling #2123S, 1:1,000), Src pY416 (Cell Signaling #6943, 1:1,000), ERK1/2 (Cell Signaling #4695, 1:2,000), ERK1/2 pT202/pY204 (Cell Signaling # 9101S, 1:2,000), Cx43 pY265 (Abcam #193373, 1:1,000), Cx43 pS279 (Invitrogen # PA5-64640, 1:2,000), Cx43 pS282 (Invitrogen # PA5-64641, 1:2,000), Cx43 (Sigma #6219, 1:10,000), Anti-rabbit IgG - HRP-linked Antibody (Cell Signaling #7074, 1:1,000 to 10,000), Anti-mouse IgG - HRP-linked Antibody (Cell Signaling #7076, 1:1,000). ..

    other:

    Article Title: A Cysteine Residue within the Kinase Domain of Zap70 Regulates Lck Activity and Proximal TCR Signaling
    Article Snippet: Src pY416 , 2101S , Cell Signaling Technology.

    Article Title: Dynamic incorporation of histone H3 variants into chromatin is essential for acquisition of aggressive traits and metastatic colonization
    Article Snippet: SRC pY416 , Cell Signaling , 6943S.

    Electrophoresis:

    Article Title: Activated Src requires Cadherin-11, Rac, and gp130 for Stat3 activation and survival of mouse Balb/c3T3 fibroblasts
    Article Snippet: .. Following electrophoresis and transfer, membranes were cut into strips based on the positions of pre-stained markers and probed for Src pY416 (Cell Signaling, Danvers, MA, cat. #2101), pan-Src (Cell Signaling, cat. #2109), Cadherin-11 (Cell Signaling, cat. #4442), Stat3-ptyr705 (Cell Signaling, cat. #9145, Rabbit) cleaved PARP (Cell Signaling, cat. #9544), Rac (Millipore #05-389), b-actin (Cell Signaling #3700) and secondary antibodies according to the manufacturer’s protocols. .. Bands were visualized using the Clarity Western ECL substrate (Bio-Rad, Hercules, CA, cat. #170-5060).

    Concentration Assay:

    Article Title: Calmodulin Directly Interacts with the Cx43 Carboxyl-Terminus and Cytoplasmic Loop Containing Three ODDD-Linked Mutants (M147T, R148Q, and T154A) that Retain α-Helical Structure, but Exhibit Loss-of-Function and Cellular Trafficking Defects
    Article Snippet: After re-plating and once colonies were formed, Western blot and immunofluorescence were used to screen the clones (α-Cx43, Sigma #6219, 1:10,000, MilliporeSigma, St. Louis, MO, USA). .. Detection reagents, manufacturers, and concentration ranges for all primary and secondary antibodies utilized in Western blot imaging are as follow: Pyk2 (Cell Signaling #3480S, 1:1,000, Danvers, USA), Pyk2 pY402 (Abcam #4800, 1:1,000, Cambridge, UK), PKCα (Cell Signaling #2056S, 1:1,000), PKCα pT638 (Abcam #32502, 1:5,000), Src (Cell Signaling #2123S, 1:1,000), Src pY416 (Cell Signaling #6943, 1:1,000), ERK1/2 (Cell Signaling #4695, 1:2,000), ERK1/2 pT202/pY204 (Cell Signaling # 9101S, 1:2,000), Cx43 pY265 (Abcam #193373, 1:1,000), Cx43 pS279 (Invitrogen # PA5-64640, 1:2,000), Cx43 pS282 (Invitrogen # PA5-64641, 1:2,000), Cx43 (Sigma #6219, 1:10,000), Anti-rabbit IgG - HRP-linked Antibody (Cell Signaling #7074, 1:1,000 to 10,000), Anti-mouse IgG - HRP-linked Antibody (Cell Signaling #7076, 1:1,000). ..

    Imaging:

    Article Title: Calmodulin Directly Interacts with the Cx43 Carboxyl-Terminus and Cytoplasmic Loop Containing Three ODDD-Linked Mutants (M147T, R148Q, and T154A) that Retain α-Helical Structure, but Exhibit Loss-of-Function and Cellular Trafficking Defects
    Article Snippet: After re-plating and once colonies were formed, Western blot and immunofluorescence were used to screen the clones (α-Cx43, Sigma #6219, 1:10,000, MilliporeSigma, St. Louis, MO, USA). .. Detection reagents, manufacturers, and concentration ranges for all primary and secondary antibodies utilized in Western blot imaging are as follow: Pyk2 (Cell Signaling #3480S, 1:1,000, Danvers, USA), Pyk2 pY402 (Abcam #4800, 1:1,000, Cambridge, UK), PKCα (Cell Signaling #2056S, 1:1,000), PKCα pT638 (Abcam #32502, 1:5,000), Src (Cell Signaling #2123S, 1:1,000), Src pY416 (Cell Signaling #6943, 1:1,000), ERK1/2 (Cell Signaling #4695, 1:2,000), ERK1/2 pT202/pY204 (Cell Signaling # 9101S, 1:2,000), Cx43 pY265 (Abcam #193373, 1:1,000), Cx43 pS279 (Invitrogen # PA5-64640, 1:2,000), Cx43 pS282 (Invitrogen # PA5-64641, 1:2,000), Cx43 (Sigma #6219, 1:10,000), Anti-rabbit IgG - HRP-linked Antibody (Cell Signaling #7074, 1:1,000 to 10,000), Anti-mouse IgG - HRP-linked Antibody (Cell Signaling #7076, 1:1,000). ..



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